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|Type:||Artigo de periódico|
|Title:||Identification and characterization of the trnS/pseudo-tRNA/nad3/rps12 gene cluster from Coix lacryma-jobi L: organization, transcription and RNA editing|
de Souza, AP
|Abstract:||During a study of mitochondrial sequence conservation between the liverwort Marchantia polymorpha and several Angiosperm species, as revealed by heterologous hybridization experiments, the trnS/psuedo-tRNA/nad3/rps12 gene cluster in Coix lacrymajobi L., an Asian grass species from the Andropogoneae, was identified using the mitochondrial probe orf 167 from M. polymorpha. The Coh gene cluster was cloned and sequenced, and its expression analyzed. The gene sequence and gene locus organization were found to be similar to the corresponding cluster in wheat and maize. Northern hybridization and reverse transcription-polymerase chain reaction analyses indicated that nad3 and rps12 genes were co-transcribed as a 1.25 kb RNA molecule. The transcript displayed 20 and six RNA edition sites, in the nad3 and rps12 genes, respectively, that changed the codon identities to amino acids, which are better conserved in different organisms. Twenty-three cDNA clones were analysed for the edition process and revealed different partial editing patterns without apparent sequential processing. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.|
NADH dehydrogenase subunit 3
Coix lacryma-jobi L.
|Editor:||Elsevier Sci Ireland Ltd|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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