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Type: Artigo de periódico
Title: Hydrogen peroxide formation in cacao tissues infected by the hemibiotrophic fungus Moniliophthora perniciosa
Author: Dias, CV
Mendes, JS
dos Santos, AC
Pirovani, CP
Gesteira, AD
Micheli, F
Gramacho, KP
Hammerstone, J
Mazzafera, P
Cascardo, JCD
Abstract: In plant pathogen interaction, the hydrogen peroxide (H(2)O(2)) may play a dual role: its accumulation inhibits the growth of biotrophic pathogens, while it could help the infection/colonization process of plant by necrotrophic pathogens. One of the possible pathways of H(2)O(2) production involves oxalic acid (Oxa) degradation by apoplastic oxalate oxidase. Here, we analyzed the production of H(2)O(2), the presence of calcium oxalate (CaOx) crystals and the content of Oxa and ascorbic acid (Asa) the main precursor of Oxa in plants in susceptible and resistant cacao (Theobroma cacao L) infected by the hemibiotrophic fungus Moniliophthora perniciosa. We also quantified the transcript level of ascorbate peroxidase (Apx), germin-like oxalate oxidase (Gip) and dehydroascorbate reductase (Dhar) by RT-qPCR. We report that the CaOx crystal amount and the H(2)O(2) levels in the two varieties present distinct temporal and genotype-dependent patterns. Susceptible variety accumulated more CaOx crystals than the resistant one, and the dissolution of these crystals occurred in the early infection steps and in the final stage of the disease in the resistant and the susceptible variety, respectively. High expression of the Glp and accumulation of Oxa were observed in the resistant variety. The content of Asa increased in the inoculated susceptible variety, but remained constant in the resistant one. The susceptible variety presented reduced Dhar expression. The role of H(2)O(2) and its formation from Oxa via Apx and Glp in resistant and susceptible variety infected by M. perniciosa were discussed. (C) 2011 Elsevier Masson SAS. All rights reserved.
Subject: Ascorbic acid
Hydrogen peroxide
Oxalic acid
Theobroma cacao
Witches' broom disease
Country: França
Editor: Elsevier France-editions Scientifiques Medicales Elsevier
Citation: Plant Physiology And Biochemistry. Elsevier France-editions Scientifiques Medicales Elsevier, v. 49, n. 8, n. 917, n. 922, 2011.
Rights: fechado
Identifier DOI: 10.1016/j.plaphy.2011.05.004
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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