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|Type:||Artigo de periódico|
|Title:||Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer|
|Abstract:||This paper describes a simple, fast and sensitive method for the determination of hydrogen peroxide in liquid samples. The system, consisting of a mini-reactor containing horseradish peroxidase (HRP) immobilised onto polyaniline coupled to a spectrophotometer, was able to determine hydrogen peroxide in the range from 55 to 2730 nmol L-1 using pyrogallol as the reduced substrate. The determinations were made using a flow injection system with a flow rate of 3.0 mL min(-1) and optical detection at 420 nm with a glass flow cell having a I mm optical path length. The system has good response repeatability (R.S.D. 0.3-2.3%), while the mini-reactor can be stored and reused with up to 1500 injections without significant reduction of enzyme activity. (c) 2005 Elsevier Ltd. All rights reserved.|
|Editor:||Elsevier Sci Ltd|
|Citation:||Process Biochemistry. Elsevier Sci Ltd, v. 40, n. 11, n. 3441, n. 3445, 2005.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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