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Type: Artigo de periódico
Title: Hydrogen peroxide detection system consisting of chemically immobilised peroxidase and spectrometer
Author: Fernandes, KF
Lima, CS
Lopes, FM
Collins, CH
Abstract: This paper describes a simple, fast and sensitive method for the determination of hydrogen peroxide in liquid samples. The system, consisting of a mini-reactor containing horseradish peroxidase (HRP) immobilised onto polyaniline coupled to a spectrophotometer, was able to determine hydrogen peroxide in the range from 55 to 2730 nmol L-1 using pyrogallol as the reduced substrate. The determinations were made using a flow injection system with a flow rate of 3.0 mL min(-1) and optical detection at 420 nm with a glass flow cell having a I mm optical path length. The system has good response repeatability (R.S.D. 0.3-2.3%), while the mini-reactor can be stored and reused with up to 1500 injections without significant reduction of enzyme activity. (c) 2005 Elsevier Ltd. All rights reserved.
Subject: spectrophotometric biosensor
hydrogen peroxide
high sensitivity
Country: Inglaterra
Editor: Elsevier Sci Ltd
Citation: Process Biochemistry. Elsevier Sci Ltd, v. 40, n. 11, n. 3441, n. 3445, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.procbio.2005.04.003
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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