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Type: Artigo de periódico
Title: Human respiratory syncytial virus N, P and M protein interactions in HEK-293T cells
Author: Oliveira, AP
Simabuco, FM
Tamura, RE
Guerrero, MC
Ribeiro, PGG
Libermann, TA
Zerbini, LF
Ventura, AM
Abstract: Characterization of Human Respiratory Syncytial Virus (HRSV) protein interactions with host cell components is crucial to devise antiviral strategies. Viral nucleoprotein, phosphoprotein and matrix protein genes were optimized for human codon usage and cloned into expression vectors. HEK-293T cells were transfected with these vectors, viral proteins were immunoprecipitated, and co-immunoprecipitated cellular proteins were identified through mass spectrometry. Cell proteins identified with higher confidence scores were probed in the immunoprecipitation using specific antibodies. The results indicate that nucleoprotein interacts with arginine methyl-transferase, methylosome protein and Hsp70. Phosphoprotein interacts with Hsp70 and tropomysin, and matrix with tropomysin and nucleophosmin. Additionally, we performed immunoprecipitation of these cellular proteins in cells infected with HRSV, followed by detection of co-immunoprecipitated viral proteins. The results indicate that these interactions also occur in the context of viral infection, and their potential contribution for a HRSV replication model is discussed. (C) 2013 Elsevier B.V. All rights reserved.
Subject: Human respiratory syncytial virus
Protein interaction
Optimized genes
Country: Holanda
Editor: Elsevier Science Bv
Citation: Virus Research. Elsevier Science Bv, v. 177, n. 1, n. 108, n. 112, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.virusres.2013.07.010
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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