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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleHuman herpesvirus 6 in oral fluids from healthy individualspt_BR
dc.contributor.authorPereira, CMpt_BR
dc.contributor.authorGasparetto, PFpt_BR
dc.contributor.authorCorrea, MEPpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorde Almeida, SPpt_BR
dc.contributor.authorBarjas-Castro, MLpt_BR
unicamp.author.emailmaluz@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, BR-13081970 Campinas, SP, Brazil Univ Estadual Campinas, Sch Dent, BR-13414900 Piracicaba, SP, Brazilpt_BR
dc.subjectHHV6pt_BR
dc.subjectexanthema subitumpt_BR
dc.subjectsalivapt_BR
dc.subjectparotid glandpt_BR
dc.subjectviral sheddingpt_BR
dc.subject.wosPolymerase-chain-reactionpt_BR
dc.subject.wosHuman Herpesvirus-6 Dnapt_BR
dc.subject.wosPeripheral-bloodpt_BR
dc.subject.wosExanthem-subitumpt_BR
dc.subject.wosSalivapt_BR
dc.subject.wosTransplantationpt_BR
dc.subject.wosAntibodypt_BR
dc.subject.wosAdultspt_BR
dc.subject.wosViruspt_BR
dc.subject.wosHhv-6pt_BR
dc.description.abstractBackground: Human herpesvirus 6 (HHV6) is the etiologic agent of exanthem subitum. The virus is latent in salivary glands and saliva is the main form of viral transmission. The objective of this study was to assess HHV6 incidence in the fluids from healthy individuals using a standardised technique for collecting and extracting viral DNA from gingival. crevicular fluid, whole saliva and parotid gland saliva. Design: Samples of oral fluids and peripheral. blood were collected from 28 blood donors and HHV6 was detected using PCR assay. Parotid gland saliva and gingival crevicular fluid were collected by endodontic paper cones in order to not contaminate these fluids with whole saliva. Results: Of the 28 donors, 20 (71.4%) presented positive results in at least one of the three oral fluids researched. Whole saliva was positive in 19 (67.8%) volunteers, while only four (14.2%) samples of gingival crevicular fluid and four of parotid gland saliva proved to be positive. Conclusions: The results suggest that HHV6 is present in the saliva of a large proportion of the healthy adult population. The use of endodontic paper cones for oral fluid collection and viral extraction was efficient, simple, cheap and painless. In spite of, the small number of cases studied it was possible to demonstrate that neither gingival crevicular fluid nor parotid gland saliva were the principal source of HHV6 in whole saliva. (C) 2004 Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofArchives Of Oral Biologypt_BR
dc.relation.ispartofabbreviationArch. Oral Biol.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculationDECpt_BR
dc.identifier.citationArchives Of Oral Biology. Pergamon-elsevier Science Ltd, v. 49, n. 12, n. 1043, n. 1046, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume49pt_BR
dc.description.issuenumber12pt_BR
dc.description.firstpage1043pt_BR
dc.description.lastpage1046pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0003-9969pt_BR
dc.identifier.wosidWOS:000225233900012pt_BR
dc.identifier.doi10.1016/j.archoralbio.2004.06.002pt_BR
dc.date.available2014-11-14T09:05:47Z
dc.date.available2015-11-26T16:05:32Z-
dc.date.accessioned2014-11-14T09:05:47Z
dc.date.accessioned2015-11-26T16:05:32Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:05:32Z (GMT). No. of bitstreams: 2 WOS000225233900012.pdf: 81996 bytes, checksum: 175f3fed2826ff569567c6666980c56c (MD5) WOS000225233900012.pdf.txt: 13678 bytes, checksum: e78c8475ad1ded74e767308d9c8a3612 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68903pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/68903
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68903-
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