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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleHRP-based amperometric biosensor for the polyphenols determination in vegetables extractpt_BR
dc.contributor.authorMello, LDpt_BR
dc.contributor.authorSotomayor, MDPTpt_BR
dc.contributor.authorKubota, LTpt_BR
unicamp.author.emailkubota@iqm.unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, Inst Quim, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectbiosensorpt_BR
dc.subjecthorseradish peroxidasept_BR
dc.subjectpolyphenolspt_BR
dc.subjectfood analysispt_BR
dc.subject.wosPhenolic-compoundspt_BR
dc.subject.wosOlive Oilspt_BR
dc.subject.wosRancidification Processpt_BR
dc.subject.wosHorseradish-peroxidasept_BR
dc.subject.wosNatural Polyphenolspt_BR
dc.subject.wosHydrogen-peroxidept_BR
dc.subject.wosAntioxidantspt_BR
dc.subject.wosElectrodespt_BR
dc.subject.wosSilicapt_BR
dc.subject.wosChemistrypt_BR
dc.description.abstractThe determination of polyphenols is important due to their biological importance as plant secondary metabolite, their ecological role in the antioxidant capacity, physiological effects, employment as markers in taxonomic studies, and their properties to food quality control. Therefore, a biosensor based on horseradish peroxidase (HRP) and DNA immobilized onto silica-titanium is presented, and its application for measuring the polyphenols compounds in vegetables samples was studied. Various analytical parameters influencing the biosensor performance have been studied as function of the chlorogenic acid (CGA) polyphenol compound, parameters such as working potential, type and concentration of the buffer, pH, response time and response with other compounds were investigated. In the optimized conditions, the biosensor presented a linear response range for CGA from 1 to 50 mumol l(-1), applying a potential of -50 mV versus Ag/AgCl, with a sensitivity of 181 mumol(-1) 1 nA cm(-2) and a detection limit of 0.7 mumol l(-1). The biosensor was tested in the polyphenols determination in vegetables extract and the results were compared with the Folin-Ciocalteau traditional method and the biosensor showed suitability to the quantification of the total polyphenol in the tested samples. (C) 2003 Elsevier B.V. All rights reserved.pt
dc.relation.ispartofSensors And Actuators B-chemicalpt_BR
dc.relation.ispartofabbreviationSens. Actuator B-Chem.pt_BR
dc.publisher.cityLausannept_BR
dc.publisher.countrySuíçapt_BR
dc.publisherElsevier Science Sapt_BR
dc.date.issued2003pt_BR
dc.date.monthofcirculationDEC 1pt_BR
dc.identifier.citationSensors And Actuators B-chemical. Elsevier Science Sa, v. 96, n. 3, n. 636, n. 645, 2003.pt_BR
dc.language.isoenpt_BR
dc.description.volume96pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage636pt_BR
dc.description.lastpage645pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0925-4005pt_BR
dc.identifier.wosidWOS:000187039100024pt_BR
dc.identifier.doi10.1016/j.snb.2003.07.008pt_BR
dc.date.available2014-11-14T14:43:21Z
dc.date.available2015-11-26T16:07:01Z-
dc.date.accessioned2014-11-14T14:43:21Z
dc.date.accessioned2015-11-26T16:07:01Z-
dc.description.provenanceMade available in DSpace on 2014-11-14T14:43:21Z (GMT). No. of bitstreams: 1 WOS000187039100024.pdf: 176090 bytes, checksum: 8126e712f8d567e37f0f7bec8162d709 (MD5) Previous issue date: 2003en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:07:01Z (GMT). No. of bitstreams: 2 WOS000187039100024.pdf: 176090 bytes, checksum: 8126e712f8d567e37f0f7bec8162d709 (MD5) WOS000187039100024.pdf.txt: 41050 bytes, checksum: 76be33e132b72c1df0a262dcfeaa2e30 (MD5) Previous issue date: 2003en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68887pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/68887
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68887-
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