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Type: Artigo de periódico
Title: HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex paraguariensis Beverages and On-Line Evaluation of Individual Antioxidant Activity
Author: Peres, RG
Tonin, FG
Tavares, MFM
Rodriguez-Amaya, DB
Abstract: Chimarrao and "terere" are mate (dried, toasted and milled Ilex paraguariensis leaves and stemlets) beverages widely consumed in South America. This paper describes the application of HPLC-DAD-ESI/MS method for the identification and quantification of caffeoylquinic acids (CQA), flavonol glycosides and purine alkaloids in these beverages. The beverage samples were prepared from commercial lots of mate from Southern Brazil. The caffeoylquinic acids, 4,5-diCQA, 3-CQA, 5-CQA, and 4-CQA were the major compounds, having 238-289, 153-242, 183-263, and 123-188 mu g/mL, respectively, for chimarrao and 206-265, 122-218, 164-209, 103-169 mu g/mL, respectively, for terere. Caffeine also had high amounts while glycosides of quercetin and kaempferol were found at much lower levels. The individual antioxidant activity was also determined by an on-line system that measured their ABTS(center dot+) radical scavenging activity, showing that the antioxidant capacity was not proportional to the concentrations of the phenolic compounds. 3-CQA, quercetina-3-O-ramnosylglucoside, and quercetina-3-O-glucoside were the major contributors to the antioxidant capacity, although the quercetin glycosides had concentrations less than 10 times that of 3-CQA.
Subject: beverage
Ilex paraguarienses
phenolic compounds
antioxidant capacity
Country: Suíça
Editor: Mdpi Ag
Citation: Molecules. Mdpi Ag, v. 18, n. 4, n. 3859, n. 3871, 2013.
Rights: aberto
Identifier DOI: 10.3390/molecules18043859
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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