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Type: Artigo de periódico
Title: Haplotype analysis and (A)gamma gene polymorphism associated with the Brazilian type of hereditary persistence of fetal hemoglobin
Author: Bordin, S
Martins, JT
Goncalves, MS
Melo, MB
Saad, STO
Costa, FF
Abstract: We have identified three unrelated individuals and three members of a family with the non-deletion form of (A) gamma-hereditary persistence of fetal hemoglobin (HPFH). Molecular analysis showed that each individual is a heterozygote for a previously described -195 (A) gamma (C-->G) mutation. The beta-globin gene cluster was studied using the polymerase chain reaction and related techniques. Haplotyping using nine restriction sites identified two closely related chromosomes with the -195 (A) gamma mutation, differing only in a single site 3' to the beta-globin gene. Further analysis of beta-globin framework indicated that the HPFH allele segregates with haplotype V, according to Orkin's classification. The second haplotype probably originated by a point mutation or DNA rearrangement of a pre-existing -195(A) gamma chromosome. We also determined the sequences from -622 to +55 bp upstream to the (A) gamma gene and part of the A(gamma) IVS-2. We found four polymorphisms associated to the -195(A) gamma promoter region. All -195(A) gamma chromosomes had a G at positions -588 and +25 relative to the (A) gamma gene. One individual was also homozygous for polymorphisms at -398 (G-->A), and another at -369 (C-->G). Cloning and sequencing of the polymorphic patterns of the 3' region of (A) gamma IVS-2 Showed that the mutated allele is linked to beta-globin chromosome a, Some correlations between chromosome characteristics and (A) gamma point mutations were also observed, (C) 1998 Wiley-Liss, Inc.
Subject: hereditary persistence of fetal hemoglobin (HPFH)
point mutation
Country: EUA
Editor: Wiley-liss
Rights: fechado
Identifier DOI: 10.1002/(SICI)1096-8652(199805)58:1<49
Date Issue: 1998
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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