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Type: Artigo de periódico
Title: GST profiling may be useful in the screening for thyroid nodule malignancy
Author: Granja, F
Morari, J
Morari, EC
Correa, LAC
Assumpcao, LVM
Ward, LS
Abstract: Screening tools are of utmost necessity in order to identify individuals at risk for thyroid nodule cancer. The polymorphic inheritance of human drug-metabolizing enzymes, such as those encoded by the Glutathione-S-Transferase (GST) system, ;plays an important role in the development of most human cancers. GSTP1 enzyme is the most important detoxification enzyme in human head and neck tissues. An aminoacid substitution (1105V) in the GSTP1 gene result in two genotypes, GSTP1AB and GSTP1BB. Those produce a variant enzyme with lower activity and less capability of effective detoxification of carcinogens than the wild type GSTP1 AA. In order to look for the influence of GSTP1 enzymes inheritance pattern on thyroid cancer risk we used a PCR-SSCP-sequencing approach to compare the genotypes of 98 malignant nodules, including 77 papillary carcinomas (PC) and 21 follicular carcinomas (FC), to 44 benign nodules and to 157 healthy control individuals. Individuals with history of previous thyroid disease, exposure to radiation and antecedents of malignancy were excluded. Patients with PC and FC showed a significant over-representation of the variants of GSTP1 allele compared to the control population (p < 0.0001). The risk for thyroid cancer in individuals with the variant GSTP1 enzymes, after adjusting for gender, age, tobacco and drugs use, increased 7,092 (CI: 2,307-21,802) and 9,625 (CI: 2.484-37.291) times for PC and FC, respectively. We suggest that GST genotype may be associated with an increased susceptibility to thyroid cancer. GSTP1 profiling from peripheral blood may be a simple and useful tool in the screening for thyroid nodule malignancy. Glutathione-S-Transferase system; GSTP; Thyroid cancer; Screening. (C) 2004 Elsevier Ltd. All rights reserved.
Subject: GST, glutathione S-transferase
GSTP1, glutathione S-transferase pi 1
GSTP1, glutathione S-transferase pi 1 locus
PCR, polymerase chain reaction
SSCP, single strand conformation polymorphism analysis
PC, papillary carcinomas
FC, follicular carcinomas
Cl, confidence interval
M, mu
T, theta
TSH, thyrotropin stimulating hormone
ECM, Elaine Cristina Morari
LVMA, Ligia Vera Montalli Assumpcao
LSW, Laura Sterian Ward
Tg, thyroglobulin
SAS, statistical analysis system
chi(2), Chi-square test
F, Fisher test
Kruskal-Wallis, OR
odds ratio
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: Cancer Letters. Elsevier Sci Ireland Ltd, v. 209, n. 2, n. 129, n. 137, 2004.
Rights: fechado
Identifier DOI: 10.1016/j.canlet.2003.12.013
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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