Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/68520
Type: Artigo de periódico
Title: Growth phase-dependent subcellular localization of nitric oxide synthase in maize cells
Author: Ribeiro, EA
Cunha, FQ
Tamashiro, WMSC
Martins, IS
Abstract: A protein band of approximately 166 kDa was detected in the soluble fraction of root tips and young leaves of maize seedlings, based on Western blot analysis using antibodies raised against mouse macrophage nitric oxide synthase (NOS) and rabbit brain NOS, NOS activity was present in these soluble fractions, as determined by L-[U-C-14]citrulline synthesis from L-[U-C-14]arginine. Immunofluorescence showed that the maize NOS protein is present in the cytosol of cells in the division zone and is translocated into the nucleus in cells in the elongation zone of maize root tips. These results indicate the existence of a NOS enzyme in maize tissues, with the localization of this protein depending on the phase of cell growth, (C) 1999 Federation of European Biochemical Societies.
Subject: plant nitric oxide synthase
root growth
immunolocalization
Zea mays
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0014-5793(99)00138-6
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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