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http://repositorio.unicamp.br/jspui/handle/REPOSIP/68506
Type: | Artigo de periódico |
Title: | Growth inhibitory effects of 3 '-nitro-3-phenylamino nor-beta-lapachone against HL-60: A redox-dependent mechanism |
Author: | Araujo, AJ de Souza, AA da Silva, EN Marinho, JDB de Moura, MABF Rocha, DD Vasconcellos, MC Costa, CO Pessoa, C de Moraes, MO Ferreira, VF de Abreu, FC Pinto, AV Montenegro, RC Costa-Lotufo, LV Goulart, MOF |
Abstract: | In this study, the cytotoxicity, genotoxicity and early ROS generation of 2,2-dimethyl-(3H)-3-(N-3'-nitrophenylamino)naphtho[1,2-b]furan-4,5-dione (QPhNO(2)) were investigated and compared with those of its precursor, nor-beta-lapachone (nor-beta), with the main goal of proposing a mechanism of antitumor action. The results were correlated with those obtained from electrochemical experiments held in protic (acetate buffer pH 4.5) and aprotic (DMF/TBABF(4)) media in the presence and absence of oxygen and with those from dsDNA biosensors and ssDNA in solution, which provided evidence of a positive interaction with DNA in the case of QPhNO(2). QPhNO(2) caused DNA fragmentation and mitochondrial depolarization and induced apoptosis/necrosis in HL-60 cells. Pre-treatment with N-acetyl-L-cysteine partially abolished the observed effects related to the QPhNO(2) treatment, including those involving apoptosis induction, indicating a partially redox-dependent mechanism. These findings point to the potential use of the combination of pharmacology and electrochemistry in medicinal chemistry. (C) 2012 Elsevier Ltd. All rights reserved. |
Subject: | Apoptosis Cytotoxicity DNA sensors Nitroquinone Nor-beta-lapachone Bioelectrochemistry |
Country: | Inglaterra |
Editor: | Pergamon-elsevier Science Ltd |
Citation: | Toxicology In Vitro. Pergamon-elsevier Science Ltd, v. 26, n. 4, n. 585, n. 594, 2012. |
Rights: | fechado |
Identifier DOI: | 10.1016/j.tiv.2012.02.003 |
Date Issue: | 2012 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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WOS000303702900006.pdf | 1.01 MB | Adobe PDF | View/Open |
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