Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/68433
Type: Artigo de periódico
Title: Grape juice concentrate prevents oxidative DNA damage in peripheral blood cells of rats subjected to a high-cholesterol diet
Author: Aguiar, O
Gollucke, APB
de Moraes, BB
Pasquini, G
Catharino, RR
Riccio, MF
Ihara, SSM
Ribeiro, DA
Abstract: The goal of the present study was to investigate whether subchronic treatment with grape juice concentrate is able to protect liver and peripheral blood cells against cholesterol-induced injury in rats. The effects of the grape juice concentrate treatment on histopathological changes, immunohistochemistry for cyclo-oxygenase-2 (COX-2), and basal and oxidative DNA damage induced by H2O2 using a single-cell gel (comet) assay were evaluated. Male Wistar rats (n 18) were divided into three groups: group 1 - negative control; group 2 - cholesterol at 1% (w/w) in their diet, treated for 5 weeks; group 3 - cholesterol at 1% in their chow, treated for 5 weeks, and grape juice concentrate at 222 mg/d in their drinking-water in the final week only. The results indicated that the treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in group 3 compared with group 2. However, grape juice concentrate was able to decrease oxidative DNA damage induced by H2O2 in peripheral blood cells, as depicted by the tail moment results. COX-2 expression in the liver did not show statistically significant differences (P>0.05) between groups. Taken together, the present results suggest that the administration of subchronic grape juice concentrate prevents oxidative DNA damage in peripheral blood cells.
Subject: Cholesterol diet
Rats
Grape juice
Cyclo-oxygenase-2
Oxidative DNA damage
Country: Inglaterra
Editor: Cambridge Univ Press
Rights: embargo
Identifier DOI: 10.1017/S0007114510004368
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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