Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/68373
Type: Artigo
Title: High throughput MS techniques for caviar lipidomics
Author: Porcari, Andréia M.
Fernandes, Gabriel D.
Belaz, Katia Roberta A.
Schwab, Nicolas V.
Santos, Vanessa G.
Alberici, Rosana M.
Gromova, Varvara A.
Eberlin, Marcos N.
Lebedev, Albert T.
Tata, Alessandra
Abstract: The lipid profile of Sturgeon roe (caviar) was monitored by matrix assisted laser desorption/ionization mass spectrometry (MALDI(+)-MS), thermal imprinting easy ambient sonic-spray ionization mass spectrometry (TI-EASI(+)-MS) and electrospray mass spectrometry (ESI(+)-MS). Freshly salted and commercially salted pasteurized caviar samples of Atlantic sturgeon (Acipenser sturio) were stored either at +4 degrees C or at room temperature for 4 weeks. The different types of chemical information achieved by these MS techniques were compared: MALDI(+)-MS detects mainly phospholipids (PL) whereas TI-EASI(+)-MS allows monitoring of both triacylglycerols (TAG) and PL. ESI(+) coupled to Fourier transform ion cyclotron resonance high resolution mass spectrometer (FT-ICR-MS) and MS/MS experiments were used to fully characterize the detected lipids, ensure the absence of oxidation products in the degradation process and confirm the high efficiency of the thermal imprinting extraction. TI-EASI(+)-MS, via a more comprehensive profiling and easier operation, has therefore been demonstrated to provide caviar lipidomic profiles and discriminate its changes as a function of storage time and temperature. The data have also confirmed that hydrolysis is the main process of lipid degradation in caviar.
The lipid profile of Sturgeon roe (caviar) was monitored by matrix assisted laser desorption/ionization mass spectrometry (MALDI(+)-MS), thermal imprinting easy ambient sonic-spray ionization mass spectrometry (TI-EASI(+)-MS) and electrospray mass spectro
Subject: Caviar
Espectrometria de massa
Lipidômica
Country: Reino Unido
Editor: Royal Society of Chemistry
Citation: Analytical Methods. Royal Soc Chemistry, v. 6, n. 8, n. 2436, n. 2443, 2014.
Rights: aberto
Fechado
Identifier DOI: 10.1039/C3AY41954A
Address: https://pubs.rsc.org/en/content/articlelanding/2014/AY/c3ay41954a
Date Issue: 2014
Appears in Collections:IQ - Artigos e Outros Documentos
FEA - Artigos e Outros Documentos

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