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Type: Artigo de periódico
Title: High performance liquid chromatography analysis of a 4-anilinoquinazoline derivative (PD153035), a specific inhibitor of the epidermal growth factor receptor tyrosine kinase, in rat plasma
Author: Rocco, SA
Velho, JA
Marin, RM
Rolim, LD
Vercesi, AE
Rittner, R
Franchini, KG
Abstract: The quinazoline derivative, 4-N-(3'-bromo-phenyl)amino-6,7-dimethoxyquinazoline (PD153035), has recently been identified as a potential drug for the treatment of proliferative disease. Here, we report a sensitive high performance liquid chromatography (HPLC)-based quantitative detection method for measurement of PD153035 levels in rat plasma. Sample pretreatment involved a two-step extraction with chloroform. The analytes were separated on a column packed with OmniSpher C18 material and eluted with acetonitrile-0.1 M ammonium acetate, pH 7.2 (70:30, v/v). The column effluent was monitored by UV detection at 330 nm. A linear response was achieved over the concentration range 0.50-100.00 muM using multilevel calibration with an internal standard. The analytical method inter- and intra-run accuracy and precision were better than +/-15%. The lower limit of quantification was 0.50 muM. The method has been applied to study the preclinical pharmacokinetics of this compound in rats. (C) 2004 Elsevier B.V. All rights reserved.
Subject: 4-anilinoquinazoline derivatives
liquid-liquid extraction
high performance liquid chromatography
Country: Holanda
Editor: Elsevier Science Bv
Citation: Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 817, n. 2, n. 297, n. 302, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2004.12.021
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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