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Type: Artigo de periódico
Title: Glutaraldehyde-treated bovine pericardium: Effects of lyophilization on cytotoxicity and residual aldehydes
Author: Maizato, MJS
Higa, OZ
Mathor, MB
Camillo, MAP
Spencer, PJ
Pitombo, RN
Zavaglia, CAC
Leirner, AA
Abstract: This work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentra- tion of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular growth. Residual aldehydes were measured by HPLC. Although both groups' eluants exhibited some cytotoxicity, the eluant from group A was less cytotoxic, with a cytotoxicity index (IC(50(%)) ) of 41%. Group B eluants all had marked cytotoxic effects; cell growth was 24.15% of the negative control at the most dilute eluant concentration (6.25%). The mean residual glutaraldehyde level was less in group A than in group B (2.36 +/- 0.11 and 9.90 +/- 3.70 g/l, respectively; n=3, P < 0.05), but residual formaldehyde levels did not differ. These results demonstrate that compared with conventional glutaraldehyde-treated bovine pericardium, lyophilized pericardium is less cytotoxic, with fewer glutaraldehyde residues.
Subject: biocompatibility
bovine pericardium
Country: EUA
Editor: Wiley-blackwell
Citation: Artificial Organs. Wiley-blackwell, v. 27, n. 8, n. 692, n. 694, 2003.
Rights: fechado
Identifier DOI: 10.1046/j.1525-1594.2003.07275.x
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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