Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/68110
Type: Artigo de periódico
Title: GLUCOSYLTRANSFERASE PRODUCTION BY KLEBSIELLA SP K18 AND CONVERSION OF SUCROSE TO PALATINOSE USING IMMOBILIZED CELLS
Author: Orsi, DC
Kawaguti, HY
Sato, HH
Abstract: The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL(-1)) was achieved using the optimized medium composed by sugar cane molasses (80 g L(-1)), bacteriological peptone (7 g L(-1)) and yeast extract (20 g L(-1)), after 8 hours of fermentation at 28 degrees C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%) were evaluated and the yield of palatinose was approximately 62.5%.
Subject: cell immobilization
glucosyltransferase
Klebsiella sp
palatinose
response surface methodology
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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