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Type: Artigo de periódico
Title: Genotyping of human cytomegalovirus using non-radioactive single-strand conformation polymorphism (SSCP) analysis
Author: de Albuquerque, DM
Costa, SCB
Abstract: Genetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant sequence or a polymorphism in a known gene. SSCP analysis has the advantage over RFLP analysis on detection of DNA polymorphisms and point mutations at a variety of positions in DNA fragments. However, the original SSCP protocols using the incorporation of radioactive label and polyacrylamide gel electrophoresis for detection are labour intensive and time-consuming. A simplified SSCP protocol is described to identify HCMV strains and the gB genotype, allowing the detection of sequence variations not residing in the endonuclease recognition sites. (C) 2003 Elsevier Science B.V. All rights reserved.
Subject: human cytomegalovirus
glycoprotein B
PCR-restriction fragment length polymorphism
PCR-single-strand conformation polymorphism
Country: Holanda
Editor: Elsevier Science Bv
Citation: Journal Of Virological Methods. Elsevier Science Bv, v. 110, n. 1, n. 25, n. 28, 2003.
Rights: fechado
Identifier DOI: 10.1016/S0166-0934(03)00094-6
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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