Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67952
Type: Artigo de periódico
Title: GENETIC-VARIABILITY IN MITOCHONDRIAL-DNA OF THE SCREWWORM, COCHLIOMYIA-HOMINIVORAX (DIPTERA, CALLIPHORIDAE), FROM BRAZIL
Author: VARGAS, MEI
ESPIN, AMLD
Abstract: Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was used to examine genetic variation and population structnre of screwworm flies in foul populations from Sao Paulo State, Brazil. The total DNA of 405 individuals was digested with 15 restriction endonucleases and probed with five cloned HindIII fragments representing the entire mitochondrial genome of Cochliomyia hominivorax. The survey revealed that four enzymes (HaeIII, HindIII, MspI, and PvuII) were suitable to detect mtDNA variation among all populations. Based on the fragment patterns obtained for these four enzymes, a total of 15 haplotypes in combination was detected. Heteroplasmic individuals for the PvuII pattern were obtained in one of the populations. The estimated average for nucleotide sequence divergence (delta) was 0.92%. The cladogram of the geographical distribution among the observed haplotypes suggests that the sampled screwworms probably belong to a single evolutionary lineage with populations interconnected by reduced gene flow.
Subject: MITOCHONDRIAL DNA
RESTRICTION FRAGMENT LENGTH POLYMORPHISM
COCHLIOMYIA HOMINIVORAX
SCREWWORM FLY
POPULATION GENETICS
Editor: Plenum Publ Corp
Rights: fechado
Identifier DOI: 10.1007/BF00553622
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOSA1995RY31100004.pdf1.01 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.