Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67938
Type: Artigo de periódico
Title: Genetic variability and chromosome-length polymorphisms of the witches' broom pathogen Crinipellis perniciosa from various plant hosts in South America
Author: Rincones, J
Mazotti, GD
Griffith, GW
Pomela, A
Figueira, A
Leal, GA
Queiroz, MV
Pereira, JF
Azevedo, RA
Pereira, GAG
Meinhardt, LW
Abstract: Crinipellis perniciosa has been classified into at least four known biotypes associated with members of unrelated plant families. In this study, genetic variability is shown for 27 C (Cacao), 4 S (Solarium), and 7 L biotype (Liana) isolates of C. perniciosa collected from different regions of Brazil and South America. The objective was to investigate the genetic variability of the pathogen in the cacao-producing region of Bahia, Brazil, and elsewhere, through microsatellite analysis, and attempt to identify possible correlations between host specificity and electrophoretic karyotypes. The PCR-banding patterns were found to vary both within and between the different biotypes, and a correlation was established between the PCR-banding patterns and the chromosomal-banding patterns of each isolate. Microsatellite and chromosomal patterns among all of the L and S biotype isolates were distinctly different from the C biotypes analysed. A higher degree of genetic and chromosomal variability was found among C biotype isolates from the Amazon in comparison with C biotype isolates from Bahia, which seems to be comprised of only two main genotypes. This finding has important implications to the current cacao-breeding programme in Brazil. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Subject: electrophoretic karyotype
intraspecific genetic variability
microsatellite primers
pulsed-field gel electrophoresis
Theobroma cacao
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.mycres.2006.05.002
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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