Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67931
Type: Artigo de periódico
Title: Genetic relatedness between oral and intestinal isolates of Porphyromonas endodontalis by analysis of random amplified polymorphic DNA
Author: Goncalves, RB
Vaisanen, ML
Van Steenbergen, TJM
Sundqvist, G
Mouton, C
Abstract: Genomic fingerprints from the DNA of 27 strains of Porphyromonas endodontalis from diverse clinical acid geographic origins were generated as random amplified polymorphic DNA (RAPD) using the technique of PCR amplification with a single primer of arbitrary sequence. Cluster analysis of the combined RAPD data obtained with three selected 9- or 10-mer-long primers identified 25 distinct RAPD types which clustered as three main groups identifying three genogroups. Genogroups I and II included exclusively P. endodontalis isolates of oral origin, while 7/9 human intestinal strains of genogroup III which linked at a similarity level of 52% constituted the most homogeneous group in our study. Genotypic diversity within P. endodontalis, as shown by RAPD analysis, suggests that the taxon is composed of two oral genogroups and one intestinal genogroup. This hypothesis remains to be confirmed. (C) Elsevier, Paris.
Subject: genotyping
Porphyromonas endodontalis
oral genogroup
intestinal genogroup
RAPD
Country: França
Editor: Editions Scientifiques Medicales Elsevier
Rights: fechado
Identifier DOI: 10.1016/S0923-2508(99)80046-1
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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