Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67842
Type: Artigo de periódico
Title: Gene expression profiles underlying alternative caste phenotypes in a highly eusocial bee, Melipona quadrifasciata
Author: Judice, CC
Carazzole, MF
Festa, F
Sogayar, MC
Hartfelder, K
Pereira, GAG
Abstract: To evaluate caste-biased gene expression in Melipona quadrifasciata, a stingless bee, we generated 1278 ESTs using Representational Difference Analysis. Most annotated sequences were similar to honey bee genes of unknown function. Only few queen-biased sequences had their putative function assigned by sequence comparison, contrasting with the worker-biased ESTs. The expression of six annotated genes connected to caste specificity was validated by real time PCR. Interestingly, queens that were developmentally induced by treatment with a juvenile hormone analogue displayed an expression profile clearly different from natural queens for this set of genes. In summary, this study represents an important first step in applying a comparative genomic approach to queen/worker polyphenism in the bee.
Subject: representational difference analysis
subtractive hybridization
stingless bee
juvenile hormone
caste development
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1111/j.1365-2583.2005.00605.x
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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