Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67829
Type: Artigo de periódico
Title: Gemella morbillorum in primary and secondary/persistent endodontic infections
Author: Gomes, BPFA
Montagner, F
Jacinto, RC
Pinheiro, ET
Zaia, AA
Ferraz, CCR
Souza, FJ
Abstract: Objective. To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections. Study design. Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers. Results. Culture and polymerase chain reaction ( PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 ( 38%) of root canal samples from primary and in 4 of 50 ( 8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 ( 82%) and 36 of 50 ( 72%) of the, respectively, primary and secondary/persistent root canal infections studied. Conclusion. Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.
Country: EUA
Editor: Mosby-elsevier
Rights: fechado
Identifier DOI: 10.1016/j.tripleo.2007.10.005
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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