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|Type:||Artigo de periódico|
|Title:||Gemella morbillorum in primary and secondary/persistent endodontic infections|
|Abstract:||Objective. To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections. Study design. Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers. Results. Culture and polymerase chain reaction ( PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 ( 38%) of root canal samples from primary and in 4 of 50 ( 8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 ( 82%) and 36 of 50 ( 72%) of the, respectively, primary and secondary/persistent root canal infections studied. Conclusion. Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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