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|Type:||Artigo de periódico|
|Title:||GAS-CHROMATOGRAPHIC METHOD FOR QUANTITATION AND CONFIRMATION OF TRICHOTHECENES IN WHEAT|
|Abstract:||A capillary gas chromatographic method with flame ionization detection was evaluated for quantitation and confirmation of 7 trichothecenes known to occur in grain crops. The method was applied to spiked and naturally contaminated wheat samples, Samples were extracted with methanol-aqueous KCI, After clarification with ammonium sulfate and partition to chloroform, a charcoal-alumina-Celite column cleanup was used, Heptafluorobutyryl derivatives of trichothecenes were quantitated with a methyl eicosanoate internal standard, Confirmation of identity was achieved by comparing the chromatographic behavior of suspected samples with those of standards after acetylation and hydrolysis for group A trichothecenes, and acetylation and reduction for group B trichothecenes. Limits of detection ranged from 0.1 to 0.5 mu g/g, Average recoveries for diacetoxyscirpenol (DAS) and nivalenol (NIV) determined at 7 levels of contamination were 88 and 93%, respectively, Average recoveries for deoxynivalenol, NIV, DAS, T2 tetraol, and T2 triol at a single level of contamination were 85, 87, 83, 87, and 91%, respectively, Relative standard deviations for determinations of naturally contaminated samples ranged from 2.8 to 12.9%, For spiked wheat samples, the relative standard deviations ranged from 4.6 to 11.9%.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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