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Type: Artigo de periódico
Title: G120K-PEG, a human GH antagonist, decreases GH signal transduction in the liver of mice
Author: Thirone, ACP
Carvalho, CRO
Saad, MJA
Abstract: After receptor binding, growth hormone (GH) induces GH receptors (GHR) dimerization and JAK2 is activated after its association with a dimerized GHR, stimulating the tyrosyl phosphorylation of insulin receptor substrate-1 (IRS-1), IRS-2 and She proteins. G120K-PEG, a GH antagonist is produced by a mutation that blocks GH action by preventing the GHR dimerization. This study shows that the inhibitory effect of G120K-PEG was maximal with a GH:G120K-PEG ratio of 1:100, as no increase in JAK2 tyrosyl phosphorylation was observed with this dose of GH. When the dose of GH was increased and with a GH:G120K-PEG ratio of 1:10 some tyrosyl phosphorylation of JAK2 could be observed. Additionally, GH-induced IRS-1, IRS-2 and SHC tyrosyl phosphorylation was inhibited similar to50% at equimolar concentrations of the antagonist of GH and almost abolished with a GH:G120K-PEG ratio of 1:100. The results clearly show that G120K-PEG inhibits GH signal transduction in mouse liver. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Subject: G120K-PEG
growth hormone signaling (mice)
Country: Irlanda
Editor: Elsevier Ireland Ltd
Citation: Molecular And Cellular Endocrinology. Elsevier Ireland Ltd, v. 192, n. 41671, n. 65, n. 74, 2002.
Rights: fechado
Identifier DOI: 10.1016/S0303-7207(02)00110-7
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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