Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67567
Type: Artigo de periódico
Title: Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation
Author: Hatanaka, E
Dermargos, A
Hirata, AE
Vinolo, MAR
Carpinelli, AR
Newsholme, P
Armelin, HA
Curi, R
Abstract: The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.
Country: EUA
Editor: Public Library Science
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0058626
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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