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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFish exposure to nano-TiO2 under different experimental conditions: Methodological aspects for nanoecotoxicology investigationspt_BR
dc.contributor.authorClemente, Zpt_BR
dc.contributor.authorCastro, VLpt_BR
dc.contributor.authorFeitosa, LOpt_BR
dc.contributor.authorLima, Rpt_BR
dc.contributor.authorJonsson, CMpt_BR
dc.contributor.authorMaia, AHNpt_BR
dc.contributor.authorFraceto, LFpt_BR
unicamp.authorClemente, Z. Fraceto, L. F. Univ Estadual Campinas, Inst Biol, Dept Biochem, BR-13083862 Campinas, SP, Brazilpt_BR
unicamp.authorClemente, Z. Castro, V. L. Jonsson, C. M. Maia, A. H. N. EMBRAPA, Lab Ecotoxicol & Biosafety, BR-13820000 Jaguariuna, SP, Brazilpt_BR
unicamp.authorFeitosa, L. O. Lima, R. Univ Fed Sao Carlos, Biotechnol & Environm Monitoring Program, BR-18052780 Sorocaba, SP, Brazilpt_BR
unicamp.authorLima, R. Univ Sorocaba, Dept Biotechnol, BR-18023000 Sorocaba, SP, Brazilpt_BR
unicamp.authorFraceto, L. F. Sao Paulo State Univ UNESP, Dept Environm Engn, BR-18087180 Sorocaba, SP, Brazilpt_BR
dc.subjectTitanium dioxidept_BR
dc.subjectUltraviolet lightpt_BR
dc.subjectOxidative stresspt_BR
dc.subject.wosTitanium-dioxide Nanoparticlespt_BR
dc.subject.wosUv-b Radiationpt_BR
dc.subject.wosOxidative Stresspt_BR
dc.subject.wosTio2 Nanoparticlespt_BR
dc.subject.wosManufactured Nanoparticlespt_BR
dc.subject.wosAggregation Kineticspt_BR
dc.description.abstractThe ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, I, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm(2)/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of acid phosphatase activity. Under visible light, there was an increase in metallothionein level in fish exposed to 1 mg/L of nano-TiO2. Under UV light, protein carbonylation was reduced in groups exposed to 1 and 10 mg/L, while nucleus alterations in erythrocytes were higher in fish exposed to 10 mg/L. As well as improving the understanding of nano-TiO2 toxicity, the findings demonstrated the importance of considering the experimental conditions in nanoecotoxicological tests. This work provides information for the development of protocols to study substances whose toxicity is affected by illumination conditions. (C) 2013 Elsevier B.V. All rights
dc.relation.ispartofScience Of The Total Environmentpt_BR
dc.relation.ispartofabbreviationSci. Total Environ.pt_BR
dc.publisherElsevier Science Bvpt_BR 1pt_BR
dc.identifier.citationScience Of The Total Environment. Elsevier Science Bv, v. 463, n. 647, n. 656, 2013.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [Proc. 2010/06226-2]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T17:39:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:48:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
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