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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFluorometric quantification of green fluorescent protein in tobacco leaf extractspt_BR
dc.contributor.authorRobic, Gpt_BR
dc.contributor.authorLacorte, Cpt_BR
dc.contributor.authorMiranda, EApt_BR
unicamp.author.emailgoran@feq.unicamp.brpt_BR
unicamp.authorRobic, Goran Miranda, Everson A. Univ Estadual Campinas, Dept Proc Biotecnol, Fac Engn Quim, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorLacorte, Cristiano EMBRAPA Recursos Genet & Biotecnol, Lab Transferencia Genes, BR-70770900 Brasilia, DF, Brazilpt_BR
dc.subjectGFPpt_BR
dc.subjectQuantificationpt_BR
dc.subjectFluorescencept_BR
dc.subjectTobaccopt_BR
dc.subject.wosGene-expressionpt_BR
dc.subject.wosPlantspt_BR
dc.subject.wosGfppt_BR
dc.subject.wosBindingpt_BR
dc.subject.wosAequoreapt_BR
dc.description.abstractThe main use of green fluorescent protein (GFP) is as a reporter system, where the existence of the protein is usually determined visually using fluorescent microscopy. Although fluorescence-based quantification of GFP is possible, background fluorescence in plants and in plant extracts was observed by our group. Another phenomenon we observed that makes quantification difficult is the increased level of GFP fluorescence in Nicotiana benthamiana leaf extracts, probably the result of dimerization of GFP molecules promoted by interaction with some component(s) of tobacco extracts. In the current work, the background fluorescence was minimized and the enhancement of GFP fluorescence in tobacco extracts was eliminated with the addition of urea to the measured solution so that a simple quantification assay for the GFP in the tobacco extracts could be established. (c) 2009 Elsevier Inc. All rights reserved.pt
dc.relation.ispartofAnalytical Biochemistrypt_BR
dc.relation.ispartofabbreviationAnal. Biochem.pt_BR
dc.publisher.citySan Diegopt_BR
dc.publisher.countryEUApt_BR
dc.publisherAcademic Press Inc Elsevier Sciencept_BR
dc.date.issued2009pt_BR
dc.date.monthofcirculationSEP 1pt_BR
dc.identifier.citationAnalytical Biochemistry. Academic Press Inc Elsevier Science, v. 392, n. 1, n. 8, n. 11, 2009.pt_BR
dc.language.isoenpt_BR
dc.description.volume392pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage8pt_BR
dc.description.lastpage11pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0003-2697pt_BR
dc.identifier.wosidWOS:000267920600002pt_BR
dc.identifier.doi10.1016/j.ab.2009.05.016pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-11-18T10:14:58Z
dc.date.available2015-11-26T16:53:52Z-
dc.date.accessioned2014-11-18T10:14:58Z
dc.date.accessioned2015-11-26T16:53:52Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T10:14:58Z (GMT). No. of bitstreams: 1 WOS000267920600002.pdf: 249048 bytes, checksum: 62bd7d47512d248842962793a26487de (MD5) Previous issue date: 2009en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:53:52Z (GMT). No. of bitstreams: 2 WOS000267920600002.pdf: 249048 bytes, checksum: 62bd7d47512d248842962793a26487de (MD5) WOS000267920600002.pdf.txt: 25211 bytes, checksum: 23f4e4b92c8239815324641b621332bb (MD5) Previous issue date: 2009en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67349pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/67349
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67349-
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