Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/67180
Type: Artigo de periódico
Title: Frequency of Shiga toxin-producing Escherichia coli (STEC) isolates among diarrheic and non-diarrheic calves in Brazil
Author: Leomil, L
Aidar-Ugrinovich, L
Guth, BEC
Irino, K
Vettorato, MP
Onuma, DL
de Castro, AFP
Abstract: The occurrence of Shiga toxin (Stx) gene sequences was examined in 344 fecal samples from diarrheic (n = 139) and non-diarrheic (n = 205) calves from 12 beef farms in Sao Paulo State, Brazil to study the prevalence of Shiga toxin-producing Escherichia coli (STEC) strains. Forty-four (12.7%) animals were found to be positive for stx. The frequency of carriage of stx was higher in diarrheic calves (28/139, 20%) than in non-diarrheic animals (16/205, 7.8%) (P < 0.001). Among the 24 STEC strains recovered from the animals, 12 isolates carried stx1, four stx2, and 8 carried both stx1 and stx2 genes. The eae and the enterohaemolysin (Ehly) gene sequences occurred at high frequencies in these STEC strains (41.6 and 50.0%, respectively). A total of 16 serotypes were identified. The serotypes O111:NM (four isolates), O111:H8 (two) and O118:H16 (one), currently described as enterohaemorrhagic E. coli (EHEC), were isolated from cattle in Brazil for the first time. These findings reinforce the importance of cattle as a reservoir of EHEC strains in Brazil. (C) 2003 Elsevier B.V. All rights reserved.
Subject: Escherichia coli
cattle-bacteria
PCR
stx genes
Ehly genes
STEC
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.vetmic.2003.08.002
Date Issue: 2003
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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