Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66846
Type: Artigo de periódico
Title: Felodipine quantification in human plasma by high-performance liquid chromatography coupled to tandem mass spectrometry
Author: Miglioranca, LH
Barrientos-Astigarraga, RE
Schug, BS
Blume, HH
Pereira, AS
De Nucci, G
Abstract: A rapid, sensitive, robust and specific method was developed for the determination and quantitation of felodipine, in human blood plasma by liquid chromatography coupled with tandem mass spectrometry using nimodipine as internal standard. Felodipine was extracted from 0.5 mL human plasma by use of a liquid/liquid procedure using diethyl ether/hexane (80/20, v/v) as eluent. The method included a chromatographic run of 5 min using a C-18 analytical column (100 mm x 4.6 mm i.d.) and the calibration curve was linear over the range from 0.02 to 10 ng mL(-1) (r(2) > 0.994). The between-run precision, determined as relative standard deviation of replicate quality controls, was 5.7% (0.06 ng mL(-1)), 7.1% (0.6 ng mL(-1)) and 6.8% (7.5 ng mL(-1)). The between-run accuracy was +/- 0.0, 2.1 and 3.1% for the above-mentioned concentrations, respectively. (C) 2004 Elsevier B.V. All rights reserved.
Subject: felodipine
antihypertensive
healthy volunteer
LC-MS/MS
bioequivalence
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2004.10.032
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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