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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleFast determination of ethambutol in pharmaceutical formulations using capillary electrophoresis with capacitively coupled contactless conductivity detectionpt_BR
dc.contributor.authorda Silva, JAFpt_BR
dc.contributor.authorde Castro, NVpt_BR
dc.contributor.authorde Jesus, DPpt_BR
dc.contributor.authorFaria, AFpt_BR
dc.contributor.authorDe Souza, MVNpt_BR
dc.contributor.authorde Oliveira, MALpt_BR
unicamp.author.emailfracassi@iqm.unicamp.brpt_BR
unicamp.authorFracassi da Silva, Jose Alberto de Castro, Nathan Vitorazzi de Jesus, Dosil Pereira Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorFracassi da Silva, Jose Alberto de Jesus, Dosil Pereira Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorFaria, Adriana F. Leal de Oliveira, Marcone Augusto Univ Fed Juiz Fora, Inst Ciencias Exatas, Dept Quim, Juiz De Fora, Brazilpt_BR
unicamp.authorDe Souza, Marcus V. N. Fundacao Oswaldo Cruz, Inst Tecnol Farmacos Far Manguinhos, Rio De Janeiro, Brazilpt_BR
dc.subjectCEpt_BR
dc.subjectContactless conductivity detectionpt_BR
dc.subjectDrug analysispt_BR
dc.subjectElectrochemical detectionpt_BR
dc.subjectTuberculosispt_BR
dc.subject.wosLiquid-chromatographypt_BR
dc.subject.wosMicrochip Electrophoresispt_BR
dc.subject.wosOptimizationpt_BR
dc.subject.wosPlasmapt_BR
dc.description.abstractA method for the determination of ethambutol (EMB), a first-line drug against tuberculosis, based on CE with capacitively coupled contactless conductivity detection is proposed. The separation of EMB and its main product of degradation were achieved in less than 3 min with a resolution of 2.0 using a BGE composed of 50 mmol/L histidine and 30 mmol/L MES, pH 6.30. By raising the pH to 8.03, the analysis time was reduced to 1.0 min, but with a significant loss of resolution (0.7). Using the best separation 2 conditions, linearity of 0.9976 (R(2) five data points), sensitivity of 1.26 x 10(-4) V min mu mol(-1) L, and LOD and quantification of 23.5 and 78.3 mu mol/L, respectively, were obtained. Recoveries at four levels of concentration ranged from 95 to 102% and the concentration range studied ranged from 100 to 500 mu mol/L. The results obtained for the determination of EMB in pharmaceutical formulations were compared with those obtained by using CE with photometric detection.pt
dc.relation.ispartofElectrophoresispt_BR
dc.relation.ispartofabbreviationElectrophoresispt_BR
dc.publisher.cityWeinheimpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherWiley-v C H Verlag Gmbhpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationElectrophoresis. Wiley-v C H Verlag Gmbh, v. 31, n. 3, n. 570, n. 574, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume31pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage570pt_BR
dc.description.lastpage574pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlpt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0173-0835pt_BR
dc.identifier.wosidWOS:000274603200017pt_BR
dc.identifier.doi10.1002/elps.200900404pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [04/09079-0, 06/07309-3]pt
dc.date.available2014-11-18T21:04:36Z
dc.date.available2015-11-26T16:59:00Z-
dc.date.accessioned2014-11-18T21:04:36Z
dc.date.accessioned2015-11-26T16:59:00Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T21:04:36Z (GMT). No. of bitstreams: 1 WOS000274603200017.pdf: 259149 bytes, checksum: a07fab233f186a04e0c5d786df70c5fe (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:59:00Z (GMT). No. of bitstreams: 2 WOS000274603200017.pdf: 259149 bytes, checksum: a07fab233f186a04e0c5d786df70c5fe (MD5) WOS000274603200017.pdf.txt: 22292 bytes, checksum: 7ba7c3ddaf0b845a80cbfe73dc76ced5 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66775pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/66775
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66775-
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