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Type: Artigo de periódico
Title: Fast determination of ethambutol in pharmaceutical formulations using capillary electrophoresis with capacitively coupled contactless conductivity detection
Author: da Silva, JAF
de Castro, NV
de Jesus, DP
Faria, AF
De Souza, MVN
de Oliveira, MAL
Abstract: A method for the determination of ethambutol (EMB), a first-line drug against tuberculosis, based on CE with capacitively coupled contactless conductivity detection is proposed. The separation of EMB and its main product of degradation were achieved in less than 3 min with a resolution of 2.0 using a BGE composed of 50 mmol/L histidine and 30 mmol/L MES, pH 6.30. By raising the pH to 8.03, the analysis time was reduced to 1.0 min, but with a significant loss of resolution (0.7). Using the best separation 2 conditions, linearity of 0.9976 (R(2) five data points), sensitivity of 1.26 x 10(-4) V min mu mol(-1) L, and LOD and quantification of 23.5 and 78.3 mu mol/L, respectively, were obtained. Recoveries at four levels of concentration ranged from 95 to 102% and the concentration range studied ranged from 100 to 500 mu mol/L. The results obtained for the determination of EMB in pharmaceutical formulations were compared with those obtained by using CE with photometric detection.
Subject: CE
Contactless conductivity detection
Drug analysis
Electrochemical detection
Country: Alemanha
Editor: Wiley-v C H Verlag Gmbh
Citation: Electrophoresis. Wiley-v C H Verlag Gmbh, v. 31, n. 3, n. 570, n. 574, 2010.
Rights: fechado
Identifier DOI: 10.1002/elps.200900404
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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