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Type: Artigo de periódico
Title: Fast analysis of beta-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column
Author: Rostagno, MA
Debien, ICN
Vardanega, R
Nogueira, GC
Barbero, GF
Meireles, MAA
Abstract: The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of a fast analysis method for beta-ecdysone in extracts of Pfaffia glomerata. A step-by-step strategy was used to optimize temperature (30-55 degrees C), flow rate (1.0-2.0 mL min(-1)), mobile phase composition (mixtures of water and methanol or acetonitrile) and equilibration time (1-5 min). A gradient method has been developed using two solvents: 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile. Optimized conditions provided a method for the separation of beta-ecdysone in approximately 2 min with a total analysis time (sample-to-sample) of 9 min, including the return to initial conditions and the re-equilibration of the column. Evaluation of chromatographic performance revealed excellent intraday and interday reproducibility (>99.5%), resolution (2.78), selectivity (1.13), and peak symmetry (1.09) while presenting low limits of detection (0.20 mg L-1) and quantitation (0.67 mg L-1). The robustness of the method has also been calculated according to the concentration/dilution of the sample. Several sample solvents were evaluated and the best chromatographic results were obtained using 80% methanol in water. Finally, the developed method was validated with different extracts of Pfaffia glomerata samples.
Country: Inglaterra
Editor: Royal Soc Chemistry
Citation: Analytical Methods. Royal Soc Chemistry, v. 6, n. 8, n. 2452, n. 2459, 2014.
Rights: aberto
Identifier DOI: 10.1039/c3ay42276c
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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