Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66660
Type: Artigo de periódico
Title: Factorial design and response surface optimization of crude violacein for Chromobacterium violaceumi production
Author: Mendes, AS
de Carvalho, JE
Duarte, MCT
Duran, N
Bruns, RE
Abstract: Initially an eleven variable, sixteen assay 2(15-11) fractional factorial design, was used to determine the key factors in the production of violacein produced by Chromobacterium violaceum, CCT 3496. Subsequently five and three factor central composite designs were executed to determine response surfaces with the aim of optimizing cellular mass and crude violacein production. The 7.5 g l(-1) dry cell mass and 0.17 g l(-1) crude violacein productions obtained with our initial culture medium were increased to 21 g l(-1) and 0.43 g l(-1), respectively, for a medium investigated in the three factor design.
Subject: Chromobacterium violaceum
experimental design
fermentation broth
medium optimization
violacein
Country: Holanda
Editor: Kluwer Academic Publ
Rights: fechado
Identifier DOI: 10.1023/A:1013734315525
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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