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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExtraction of tetracyclinic antibiotic residues from fish filet: Comparison and optimization of different procedures using liquid chromatography with fluorescence detectionpt_BR
dc.contributor.authorOrlando, EApt_BR
dc.contributor.authorSimionato, AVCpt_BR
unicamp.authorOrlando, Eduardo Adilson Simionato, Ana Valeria C. Univ Campinas Unicamp, Inst Chem, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectFactorial experimental designpt_BR
dc.subject.wosSolid-phase Extractionpt_BR
dc.description.abstractAnti-microbials have been used to control the quality of the aquatic environment for both prophylactic and therapeutic purposes. Tetracyclines are among the main antimicrobials used in aquaculture, and present a particular difficulty for extraction, due to a complex structure and high interaction with components of the biological matrix. In this study, different techniques of extraction and clean-up of antimicrobials of the tetracycline class in tilapia filets have been optimized and compared, followed by validation of the methodology using the best procedure. Oxytetracycline, doxycycline, tetracycline and chlortetracycline were analyzed by HPLC-fluorescence under the following conditions: organic mobile phase composed of methanol:acetonitrile (1:1, v/v) and aqueous mobile phase containing sodium acetate (0.0375 mol L-1), calcium chloride (0.0175 mol L-1) and EDTA (0.0125 mol L-1) at pH 7.00. The chromatographic analysis was performed using a mobile phase gradient with a flow rate of 1 mL min(-1) and, detection wavelength of 385/528 nm (lambda(exc)/lambda(em)) Four extraction methods have been evaluated, namely: liquid-liquid partition; solid phase extraction (SPE) using phenyl, C18 and polymeric Oasis-HLB stationary phases; dispersive SPE (dSPE) using polymeric adsorbent XAD 16 resin; and QuEChERS. The methods have been optimized with fractional factorial experimental design and compared by the extraction efficiency. The liquid-liquid extraction and the QuEChERS methods showed low extraction efficiencies (14-30%) for the analytes. The use of dSPE showed good efficiency (40-60%), but with low precision and high consumption of time. Among the evaluated extraction techniques the use of SPE showed the best results, with emphasis on the phenyl phase (58-76%), and has been validated for analysis of residues of tetracyclines in tilapia muscle regarding selectivity, linearity, precision and limits of detection and quantification. The validated method was adequate for the investigation of the analytes at residue levels. (C) 2013 Elsevier B.V. All rights
dc.relation.ispartofJournal Of Chromatography Apt_BR
dc.relation.ispartofabbreviationJ. Chromatogr. Apt_BR
dc.publisherElsevier Science Bvpt_BR 13pt_BR
dc.identifier.citationJournal Of Chromatography A. Elsevier Science Bv, v. 1307, n. 111, n. 118, 2013.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.provenanceMade available in DSpace on 2014-07-30T17:34:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:42:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
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