Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66590
Type: Artigo de periódico
Title: Extraction of recombinant cytochrome b(5) from disrupted Escherichia coli cells with an aqueous two-phase system in a continuous perforated rotating disc contactor
Author: Porto, ALF
LimaFilho, JL
AiresBarros, MR
Cabral, JMS
Tambourgi, EB
Abstract: A continuous perforated rotating disc contactor was used to extract recombinant cytochrome b(5) from disrupted Escherichia coli cells with an aqueous two-phase system. About 4.9 mg (44%) total protein and 5 mg (75%) cytochrome b(5) were recovered to the salt-rich phase, using PEG (18% w/w)-KH2PO4/K2HPO4 (16% w/w) salt system. The dispersed phase (PEG) hold up increased with increasing rotor velocity. The mass transfer coefficient and separation efficiency for cytochrome, normalized for the PEG hold up, depended upon the disc's rotation speed.
Country: Holanda
Editor: Springer
Rights: fechado
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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