Please use this identifier to cite or link to this item:
|Type:||Artigo de periódico|
|Title:||Extraction of recombinant cytochrome b(5) from disrupted Escherichia coli cells with an aqueous two-phase system in a continuous perforated rotating disc contactor|
|Abstract:||A continuous perforated rotating disc contactor was used to extract recombinant cytochrome b(5) from disrupted Escherichia coli cells with an aqueous two-phase system. About 4.9 mg (44%) total protein and 5 mg (75%) cytochrome b(5) were recovered to the salt-rich phase, using PEG (18% w/w)-KH2PO4/K2HPO4 (16% w/w) salt system. The dispersed phase (PEG) hold up increased with increasing rotor velocity. The mass transfer coefficient and separation efficiency for cytochrome, normalized for the PEG hold up, depended upon the disc's rotation speed.|
|Citation:||Biotechnology Techniques. Springer, v. 11, n. 9, n. 641, n. 643, 1997.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.