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Type: Artigo de periódico
Author: Dropa, M
Balsalobre, LC
Lincopan, N
Mamizuka, EM
Murakami, T
Cassettari, VC
Franco, F
Guida, SM
Balabakis, AJ
Passadore, LF
Santos, SR
Matte, GR
Matte, MH
Abstract: Extended-spectrum beta-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and Outpatients at a public teaching hospital at Sao Paulo, Brazil, were Submitted to analysis by PCR with specific primers for bla(SHV), bla(TEM) and bla(CTX-M) genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. Bla(SHV), bla(TEM), and bla(CTX-M) were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field get eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results Suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, Suggesting clonal spread in the institutional environment.
Subject: ESBL
Klebsiella pneumoniae
Pulsed Field Gel Electrophoresis
Antimicrobial resistance
Country: Brasil
Editor: Inst Medicina Tropical Sao Paulo
Citation: Revista Do Instituto De Medicina Tropical De Sao Paulo. Inst Medicina Tropical Sao Paulo, v. 51, n. 4, n. 203, n. 209, 2009.
Rights: aberto
Identifier DOI: 10.1590/S0036-46652009000400005
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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