Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/665
Type: Artigo de periódico
Title: Assessment of the Expression of IR beta, IRS-1, IRS-2 and IGF-IR beta in a Rat Model of Intrauterine Growth Restriction
Author: BUENO, Marcia Pereira
GUADAGNINI, Dioze
GONCALVES, Frances Lilian Lanhellas
BARINI, Ricardo
SAAD, Mario Jose Abdalla
SCHMIDT, Augusto Frederico
SBRAGIA, Lourenco
Abstract: Objective: To investigate glomerular development and expression of insulin and insulin-like growth factor receptors in an experimental model of intrauterine growth restriction (IUGR). Material and Methods: We studied three groups of Sprague-Dawley fetuses: IUGR - restricted by ligation of the right uterine artery; C-IUGR - left horn controls, and EC - external controls (non-manipulated). Body and organs were weighed, and glomerular number and volume were analyzed. Expression of IR beta, IRS-1, IRS-2 and IGF-IR beta was analyzed in liver, intestine and kidneys by immunoblotting. Results: Organ/body weight ratios were similar. In IUGR, glomerular number and volume were increased compared to C-IUGR and EC (p < 0.001). In the IUGR liver, increases were found in IGF-IR beta compared to C-IUGR and EC; IR beta compared to EC, and IRS-2 compared to C-IUGR. However, decreases in IR beta were noted in IUGR compared to C-IUGR; IRS-1 compared to C-IUGR and EC, and IRS-2 compared to EC. In IUGR intestine, increases were detected in IR beta, IRS-1 and IGF-IR beta compared to C-IUGR and EC. In IUGR kidneys, increases were observed in IR beta and IGF-IR beta compared to C-IUGR and EC, and IRS-1 compared to EC. Decreased IRS-2 in the intestine and kidney were noticed in IUGR compared to C-IUGR and EC. Conclusion: IUGR fetuses had less glomeruli and alterations in insulin receptors, which may be associated with an increased risk of disease occurrence in adulthood. Copyright (C) 2010 S. Karger AG, Basel
Subject: Fetal glomerular development
Glomeruli
Insulin receptor
Insulin-like growth factor receptor
Intrauterine growth restriction
Country: Suiça
Editor: KARGER
Citation: FETAL DIAGNOSIS AND THERAPY, v.28, n.3, p.145-152, 2010
Rights: fechado
Identifier DOI: 10.1159/000316932
Address: http://dx.doi.org/10.1159/000316932
http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000283549000003&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
Date Issue: 2010
Appears in Collections:FCM - Artigos e Materiais de Revistas Científicas

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