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Type: Artigo de periódico
Title: Expression of hypoxia-inducible factor 1 alpha in mononuclear phagocytes infected with Leishmania amazonensis
Author: Degrossoli, A
Bosetto, MC
Lima, CBC
Giorgio, S
Abstract: Increasing evidence indicates that hypoxia-inducible factor 1 alpha (HIF-1 alpha) can be upregulated indifferent cell types by nonhypoxic stimuli such as growth factors, cytokines, nitric oxide, lipopolysaccharides and a range of infectious microorganisms. In this study, the ability of the following mononuclear phagocytes to express HIF-1 alpha is reported: mouse macrophages (mM Phi)), human macrophages (W(D) and human dendritic cells (DC), parasitized in vitro with Leishmania amazonensis; as assessed by immunofluorescence microscopy. A logical explanation for HIF-1 alpha expression might be that the mononuclear phagocytes became hypoxic after L. amazonensis infection. Using the hypoxia marker pimonidazole, observation revealed that L. amazonensis-infected cells were not hypoxic. In addition, experiments using a HIF-1 alpha inhibitor, CdCl2, to treat L. amazonensis-infected macrophage cultures showed reduced parasite survival. These studies indicated that HIF-1 alpha could play a role in adaptative and immune responses of mononuclear phagocytes presenting infection by the parasite L. amazonensis. (c) 2007 Elsevier B.V. All rights reserved.
Subject: hypoxia-inducible factor 1 alpha
mononuclear phagocytes
Leishmania amazonensis
Country: Holanda
Editor: Elsevier Science Bv
Citation: Immunology Letters. Elsevier Science Bv, v. 114, n. 2, n. 119, n. 125, 2007.
Rights: fechado
Identifier DOI: 10.1016/j.imlet.2007.09.009
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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