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Type: Artigo de periódico
Title: Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants
Author: Leite, A
Kemper, EL
da Silva, MJ
Luchessi, AD
Siloto, RMP
Bonaccorsi, ED
El-Dorry, HF
Arruda, P
Abstract: Human growth hormone was expressed in transgenic tobacco seeds using the monocot tissue-specific promoter from sorghum gamma-kafirin seed storage protein gene. During tobacco seed ripening, the expressed hormone was directed to the endoplasmic reticulum by a signal peptide from a Coix prolamin and was secreted into the apoplastic space, where it accounted for 0.16% of total soluble seed protein. The expressed hormone has the same amino acid sequence and receptor-binding properties as the native mature hormone.
Subject: seed-specific expression
human growth hormone
plant farming
heterologous protein
transgenic plants
Country: Holanda
Editor: Kluwer Academic Publ
Citation: Molecular Breeding. Kluwer Academic Publ, v. 6, n. 1, n. 47, n. 53, 2000.
Rights: fechado
Identifier DOI: 10.1023/A:1009648532210
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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