Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66209
Type: Artigo de periódico
Title: New insights on proteomics of transgenic soybean seeds: evaluation of differential expressions of enzymes and proteins
Author: Barbosa, HS
Arruda, SCC
Azevedo, RA
Arruda, MAZ
Abstract: This work reports the evaluation of differentially expressed enzymes and proteins from transgenic and nontransgenic soybean seeds. Analysis of malondialdehyde, ascorbate peroxidase (EC 1.11.1.11), glutathione reductase (EC 1.6.4.2), and catalase (EC 1.11.1.6) revealed higher levels (29.8, 30.6, 71.4, and 35.3%, respectively) in transgenic seeds than in nontransgenic seeds. Separation of soybean seed proteins was done by two-dimensional polyacrylamide gel electrophoresis, and 192 proteins were identified by matrix-assisted laser desorption/ionization (MALDI) quadrupole time-of-flight (QTOF) mass spectrometry (MS) and electrospray ionization (ESI) QTOF MS. Additionally, the enzyme CP4 EPSPS, involved in the genetic modification, was identified by enzymatic digestions using either trypsin or chymotrypsin and ESI-QTOF MS/MS for identification. From the proteins identified, actin fragment, cytosolic glutamine synthetase, glycinin subunit G1, and glycine-rich RNA-binding protein were shown to be differentially expressed after analysis using the two-dimensional difference gel electrophoresis technique, and applying a regulator factor of 1.5 or greater.
Subject: Two-dimensional difference gel electrophoresis
Mass spectrometry
Reactive oxygen species
Genetically modified organisms
Country: Alemanha
Editor: Springer Heidelberg
Rights: fechado
Identifier DOI: 10.1007/s00216-011-5409-1
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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