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|Type:||Artigo de periódico|
|Title:||Ex vivo antimicrobial activity of several bleaching agents used during the walking bleach technique|
|Abstract:||To investigate ex vivo the antimicrobial activity of a paste of sodium perborate associated with various vehicles comparing it with 37% carbamide peroxide and 35% hydrogen peroxide. The antimicrobial activity of these agents was evaluated against three microorganisms: Enterococcus faecalis, Streptococcus mutans and Candida albicans. One millilitre of each tested substance was placed on the bottom of wells of 24-well cell culture plates. Six wells were used for each time period and group. Two millilitres of the microbial suspension was ultrasonically mixed for 10 s with the bleaching pastes and placed in contact with them for 10, 30, 45 s; 1, 3, 5, 10, 20, 30 min; and 1 and 2 h. After each period of time, 1 mL from each well was transferred to tubes containing 2 mL of freshly prepared brain heart infusion agar + neutralizers. Agar plates were inoculated in appropriate gaseous conditions. Data were analysed statistically by the Kruskal-Wallis test with the level of significance set at P < 0.05. In all groups containing chlorhexidine (groups 3, 5 and 7), the antimicrobial activity of the bleaching paste was significantly increased when compared with groups with other kinds of vehicle (groups 1, 2, 4, 6 and 8). For all tested groups, the most resistant microorganism was E. faecalis. Chlorhexidine when used as a vehicle for sodium perborate enhanced its antimicrobial activity.|
|Editor:||Wiley-blackwell Publishing, Inc|
|Citation:||International Endodontic Journal. Wiley-blackwell Publishing, Inc, v. 41, n. 12, n. 1054, n. 1058, 2008.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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