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Type: Artigo de periódico
Title: Ex vivo antimicrobial activity of several bleaching agents used during the walking bleach technique
Author: Oliveira, DP
Gomes, BPFA
Zaia, AA
Souza, FJ
Ferraz, CCR
Abstract: To investigate ex vivo the antimicrobial activity of a paste of sodium perborate associated with various vehicles comparing it with 37% carbamide peroxide and 35% hydrogen peroxide. The antimicrobial activity of these agents was evaluated against three microorganisms: Enterococcus faecalis, Streptococcus mutans and Candida albicans. One millilitre of each tested substance was placed on the bottom of wells of 24-well cell culture plates. Six wells were used for each time period and group. Two millilitres of the microbial suspension was ultrasonically mixed for 10 s with the bleaching pastes and placed in contact with them for 10, 30, 45 s; 1, 3, 5, 10, 20, 30 min; and 1 and 2 h. After each period of time, 1 mL from each well was transferred to tubes containing 2 mL of freshly prepared brain heart infusion agar + neutralizers. Agar plates were inoculated in appropriate gaseous conditions. Data were analysed statistically by the Kruskal-Wallis test with the level of significance set at P < 0.05. In all groups containing chlorhexidine (groups 3, 5 and 7), the antimicrobial activity of the bleaching paste was significantly increased when compared with groups with other kinds of vehicle (groups 1, 2, 4, 6 and 8). For all tested groups, the most resistant microorganism was E. faecalis. Chlorhexidine when used as a vehicle for sodium perborate enhanced its antimicrobial activity.
Subject: antimicrobial activity
bleaching agents
2% chlorhexidine
Country: EUA
Editor: Wiley-blackwell Publishing, Inc
Citation: International Endodontic Journal. Wiley-blackwell Publishing, Inc, v. 41, n. 12, n. 1054, n. 1058, 2008.
Rights: fechado
Identifier DOI: 10.1111/j.1365-2591.2008.01448.x
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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