Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66182
Type: Artigo de periódico
Title: Evolutionary and structural analysis of the cytochrome c oxidase subunit I (COI) gene from Haematobia irritans, Stomoxys calcitrans and Musca domestica (Diptera : Muscidae) mitochondrial DNA
Author: De Oliveira, MT
De Azeredo-Espin, AML
Lessinger, AC
Abstract: This work describes the molecular characterization of the cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA from three species of great medical and veterinary importance: the horn fly, Haematobia irritans, the stable fly, Stomoxys calcitrans and the house fly, Musca domestica (Diptera: Muscidae) (Linnaeus). The nucleotide sequence in all species was 1536 bp in size and coded for a 512 amino acid peptide. The nucleotide bias for an A + T-rich sequence is linked to three features: a high A + T content throughout the entire gene, a high A + T content in the third codon position, and a predominance of A + T-rich codons. An anomalous TCG (serine) start codon was identified. Comparative analysis among members of the Muscidae, Scatophagidae, Calliphoridae and Drosophilidae showed high levels of nucleotide sequence conservation. Analysis of the divergent amino acids and COT protein topologies among these three Muscidae species agreed with the evolutionary model suggested for the insect mitochondrial COT protein. The characterization of the structure and evolution of this gene could be informative for further evolutionary analysis of dipteran species.
Subject: mitochondrial DNA
COI
molecular characterization
horn fly
stable fly
house fly
Country: Inglaterra
Editor: Taylor & Francis Ltd
Rights: fechado
Identifier DOI: 10.1080/10425170500039901
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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