Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/661
Type: Artigo de periódico
Title: Protective efficacy of different strategies employing Mycobacterium leprae heat-shock protein 65 against tuberculosis
Author: SOUZA, Patricia R. M.
ZARATE-BLADES, Carlos R.
HORI, Juliana I.
RAMOS, Simone G.
LIMA, Deison S.
SCHNEIDER, Tatiana
ROSADA, Rogerio S.
TORRE, Lucimara G. L.
SANTANA, Maria Helena A.
BRANDAO, Izaira T.
MASSON, Ana P.
COELHO-CASTELO, Arlete A. M.
BONATO, Vania L.
GALETTI, Fabio C. S.
GONCALVES, Eduardo D.
BOTTE, Domingos A.
MACHADO, Jeanne B. M.
SILVA, Celio L.
Abstract: Background: Tuberculosis is a major threat to human health. The high disease burden remains unaffected and the appearance of extremely drug-resistant strains in different parts of the world argues in favor of the urgent need for a new effective vaccine. One of the promising candidates is heat-shock protein 65 when used as a genetic vaccine (DNAhsp65). Nonetheless, there are substantial data indicating that BCG, the only available anti-TB vaccine for clinical use, provides other important beneficial effects in immunized infants. Methods: We compared the protective efficacy of BCG and Hsp65 antigens in mice using different strategies: i) BCG, single dose subcutaneously; ii) naked DNAhsp65, four doses, intramuscularly; iii) liposomes containing DNAhsp65, single dose, intranasally; iv) microspheres containing DNAhsp65 or rHsp65, single dose, intramuscularly; and v) prime-boost with subcutaneous BCG and intramuscular DNAhsp65. Results: All the immunization protocols were able to protect mice against infection, with special benefits provided by DNAhsp65 in liposomes and prime-boost strategies. Conclusion: Among the immunization protocols tested, liposomes containing DNAhsp65 represent the most promising strategy for the development of a new anti-TB vaccine.
Subject: heat-shock protein
liposome
prime-booster
tuberculosis
vaccine
Country: Inglaterra
Editor: INFORMA HEALTHCARE
Rights: fechado
Identifier DOI: 10.1517/14712598.8.9.1255
Address: http://dx.doi.org/10.1517/14712598.8.9.1255
http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000259213000002&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
Date Issue: 2008
Appears in Collections:FEQ - Artigos e Outros Documentos

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