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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEvidence of Chagas disease in seronegative Brazilian patients with megaesophaguspt_BR
dc.contributor.authorBatista, AMpt_BR
dc.contributor.authorAguiar, Cpt_BR
dc.contributor.authorAlmeida, EApt_BR
dc.contributor.authorGuariento, MEpt_BR
dc.contributor.authorWanderley, JSpt_BR
dc.contributor.authorCosta, SCBpt_BR
unicamp.author.emailcosta@fcm.unicamp.brpt_BR
unicamp.authorBatista, Angelica M. Aguiar, Camila Almeida, Eros A. Guariento, Maria E. Wanderley, Jamiro S. Costa, Sandra C. B. Univ Estadual Campinas UNICAMP, Fac Ciencias Med, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectChagas diseasept_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectPolymerase chain reactionpt_BR
dc.subjectEsophageal motility disorderspt_BR
dc.subject.wosPolymerase-chain-reactionpt_BR
dc.subject.wosDetect Trypanosoma-cruzipt_BR
dc.subject.wosKinetoplast Dnapt_BR
dc.subject.wosParasite Detectionpt_BR
dc.subject.wosBlood-samplespt_BR
dc.subject.wosDiagnosispt_BR
dc.subject.wosAmplificationpt_BR
dc.subject.wosPcrpt_BR
dc.subject.wosXenodiagnosispt_BR
dc.subject.wosInfectionpt_BR
dc.description.abstractBackground: After 100 years of research, Chagas disease (CD) remains an important public health problem in Latin America. The symptomatic chronic phase is usually characterized by cardiac or digestive involvement and diagnosis currently relies on the measurement of Trypanosoma cruzi-specific antibodies produced in response to the infection. However, the detection of parasite DNA in seronegative persons has been reported. Methods: The prevalence of CD in a population with esophageal disorders was assessed by conventional serology. We also detected T. cruzi DNA in blood samples of seronegative and inconclusive patients by nested polymerase chain reaction (N-PCR). Results: The seroprevalence of CD determined by conventional serologic tests (indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA)) was 79% in 513 patients with esophageal disorders. Out of 41 blood samples, N-PCR was positive in 31 (76%) cases for which serology was negative or inconclusive. Conclusions: As all patients presented with clinical signs suggestive of the digestive form of CD and most of them were born in endemic areas, we highlight the importance of improving diagnosis of the disease and the implications for blood bank screening. Our data suggest that N-PCR is effective in the detection of T. cruzi DNA in patients with inconclusive or negative serology, and it may eventually be useful in the determination of the etiology of megaesophagus. (C) 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofInternational Journal Of Infectious Diseasespt_BR
dc.relation.ispartofabbreviationInt. J. Infect. Dis.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherElsevier Sci Ltdpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationNOVpt_BR
dc.identifier.citationInternational Journal Of Infectious Diseases. Elsevier Sci Ltd, v. 14, n. 11, n. E974, n. E977, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume14pt_BR
dc.description.issuenumber11pt_BR
dc.description.firstpageE974pt_BR
dc.description.lastpageE977pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1201-9712pt_BR
dc.identifier.wosidWOS:000284059300005pt_BR
dc.identifier.doi10.1016/j.ijid.2010.05.017pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFAEPEXpt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-11-17T09:38:43Z
dc.date.available2015-11-26T17:22:35Z-
dc.date.accessioned2014-11-17T09:38:43Z
dc.date.accessioned2015-11-26T17:22:35Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:22:35Z (GMT). No. of bitstreams: 2 WOS000284059300005.pdf: 120649 bytes, checksum: 3fbaad9195e5e65555fe7dd7152808ce (MD5) WOS000284059300005.pdf.txt: 27314 bytes, checksum: 6c5cb6b9c9a28fa981f077a4e1a02efc (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66098pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/66098
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66098-
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