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Type: Artigo de periódico
Title: Evidence for the interaction of the regulatory protein Ki-1/57 with p53 and its interacting proteins
Author: Nery, FC
Rui, E
Kuniyoshi, TM
Kobarg, J
Abstract: Ki-1/57 is a cytoplasmic and nuclear phospho-protein of 57 kDa and interacts with the adaptor protein RACK1, the transcription factor MEF2C, and the chromatin remodeling factor CHD3, suggesting that it might be involved in the regulation of transcription. Here, we describe yeast two-hybrid studies that identified a total of 11 proteins interacting with Ki-1/57, all of which interact or are functionally associated with p53 or other members of the p53 family of proteins. We further found that Ki-1/57 is able to interact with p53 itself in the yeast two-hybrid system when the interaction was tested directly. This interaction could be confirmed by pull down assays with purified proteins in vitro and by reciprocal co-immunoprecipitation assays from the human Hodgkin analogous lymphoma cell line L540. Furthermore, we found that the phosphorylation of p53 by PKC abolishes its interaction with Ki-1/57 in vitro. (c) 2006 Elsevier Inc. All rights reserved.
Subject: protein protein interaction
yeast two-hybrid system
cellular localization
protein interaction network
Country: EUA
Editor: Academic Press Inc Elsevier Science
Citation: Biochemical And Biophysical Research Communications. Academic Press Inc Elsevier Science, v. 341, n. 3, n. 847, n. 855, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.bbrc.2006.01.036
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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