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Type: Artigo de periódico
Title: Evidence for increased expression of tissue factor and protease-activated receptor-1 in human esophageal cancer
Author: Ribeiro, FS
Simao, TA
Amoedo, ND
Andreollo, NA
Lopes, LR
Acatauass, R
Rumjanek, FD
Albano, RM
Pinto, LFR
Monteiro, RQ
Abstract: It has been suggested that the blood clotting initiator protein, tissue factor (TF), participates in tumor growth, metastasis and angiogenesis. In addition, a family of G protein-coupled-receptors known as protease-activated receptors (PARs) has also been implicated in tumor biology. These receptors might be activated by blood coagulation proteases thus eliciting a number of pro-tumoral responses, including the expression of interleukin-8 (IL-8). Therefore, in this study we analyzed the expression of TF, PAR-1, PAR-2 and IL-8 genes in patients with esophageal cancer, one of the most aggressive neoplastic diseases. Total RNA was extracted from tissue samples (tumor and the corresponding normal mucosa) obtained from patients submitted to esophagectomy or endoscopy and further analyzed by semi-quantitative reverse transcriptase-polymerase (RT-PCR) and/or real-time quantitative PCR (qPCR). Expression of full-length transmembrane TF was significantly higher in tumor samples whereas no differences were observed in alternatively spliced TF transcripts. Tumor tissue showed increased mRNA levels for PAR-1 but not PAR-2. Remarkably, IL-8 expression was not detected in most normal tissues but showed very high expression in tumor samples. As expected, qPCR revealed greater differences in the expression pattern of all transcripts analyzed but the general profile was very similar to that observed by RT-PCR. Altogether our data suggest a possible role for blood clotting proteins in the biology of human esophageal cancer.
Subject: esophageal squamous cell carcinoma
clotting initiator tissue factor
protease activated receptor-1
Country: Grécia
Editor: Spandidos Publ Ltd
Citation: Oncology Reports. Spandidos Publ Ltd, v. 21, n. 6, n. 1599, n. 1604, 2009.
Rights: embargo
Identifier DOI: 10.3892/or_00000393
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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