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Type: Artigo de periódico
Title: Evidence for glycosylation on a DNA-binding protein of Salmonella enterica
Author: Hanna, ES
Roque-Barreira, MC
Bernardes, ES
Panunto-Castelo, A
Sousa, MV
Almeida, IC
Brocchi, M
Abstract: Background: All organisms living under aerobic atmosphere have powerful mechanisms that confer their macromolecules protection against oxygen reactive species. Microorganisms have developed biomolecule-protecting systems in response to starvation and/or oxidative stress, such as DNA biocrystallization with Dps ( DNA-binding protein from starved cells). Dps is a protein that is produced in large amounts when the bacterial cell faces harm, which results in DNA protection. In this work, we evaluated the glycosylation in the Dps extracted from Salmonella enterica serovar Typhimurium. This Dps was purified from the crude extract as an 18-kDa protein, by means of affinity chromatography on an immobilized jacalin column. Results: The N-terminal sequencing of the jacalin-bound protein revealed 100% identity with the Dps of S. enterica serovar Typhimurium. Methyl-alpha-galactopyranoside inhibited the binding of Dps to jacalin in an enzyme-linked lectin assay, suggesting that the carbohydrate recognition domain ( CRD) of jacalin is involved in the interaction with Dps. Furthermore, monosaccharide compositional analysis showed that Dps contained mannose, glucose, and an unknown sugar residue. Finally, jacalin-binding Dps was detected in larger amounts during the bacterial earlier growth periods, whereas high detection of total Dps was verified throughout the bacterial growth period. Conclusion: Taken together, these results indicate that Dps undergoes post-translational modifications in the pre- and early stationary phases of bacterial growth. There is also evidence that a small mannose-containing oligosaccharide is linked to this bacterial protein.
Country: Inglaterra
Editor: Biomed Central Ltd
Citation: Microbial Cell Factories. Biomed Central Ltd, v. 6, 2007.
Rights: aberto
Identifier DOI: 10.1186/1475-2859-6-11
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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