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Type: Artigo de periódico
Title: Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: General implications and practical use in endodontic antimicrobial therapy
Author: Horz, HP
Vianna, ME
Gomes, BPFA
Conrads, G
Abstract: By reexamining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.
Country: EUA
Editor: Amer Soc Microbiology
Citation: Journal Of Clinical Microbiology. Amer Soc Microbiology, v. 43, n. 10, n. 5332, n. 5337, 2005.
Rights: aberto
Identifier DOI: 10.1128/JCM.43.10.5332-5337.2005
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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