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|Type:||Artigo de periódico|
|Title:||Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: Precipitation of porcine trypsin|
|Abstract:||In this work, the precipitation of porcine trypsin in solutions of volatile electrolytes formed by ammonia and carbon dioxide was investigated. The solubility curve of trypsin at 4 degrees C was determined by measuring the compositions of the precipitate and supernatant phases. Analysis showed that ammonium carbamate is able to induce trypsin salting-out. The verified salting-out property of ammonium carbamate, the easy removal of the salt out of the protein precipitate and mother-solution and the high activity recovery demonstrate the feasibility of using ammonium carbarnate as precipitant agent. (c) 2006 Elsevier B.V. All rights reserved.|
|Editor:||Elsevier Science Sa|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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