Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/66023
Type: Artigo de periódico
Title: Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: Precipitation of porcine trypsin
Author: Watanabe, EO
Pessoa, PD
Miranda, EA
Mohamed, RS
Abstract: In this work, the precipitation of porcine trypsin in solutions of volatile electrolytes formed by ammonia and carbon dioxide was investigated. The solubility curve of trypsin at 4 degrees C was determined by measuring the compositions of the precipitate and supernatant phases. Analysis showed that ammonium carbamate is able to induce trypsin salting-out. The verified salting-out property of ammonium carbamate, the easy removal of the salt out of the protein precipitate and mother-solution and the high activity recovery demonstrate the feasibility of using ammonium carbarnate as precipitant agent. (c) 2006 Elsevier B.V. All rights reserved.
Subject: downstream processing
enzyme activity
protease
separation
volatile electrolyte
trypsin
Country: Suíça
Editor: Elsevier Science Sa
Rights: fechado
Identifier DOI: 10.1016/j.bej.2006.02.013
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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