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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEvaluation of the internal transcribed spacer 2 (ITS2) as a molecular marker for phylogenetic inference using sequence and secondary structure information in blow flies (Diptera: Calliphoridae)pt_BR
dc.contributor.authorMarinho, MATpt_BR
dc.contributor.authorJunqueira, ACMpt_BR
dc.contributor.authorAzeredo-Espin, AMLpt_BR
unicamp.authorMarinho, M. A. T. Junqueira, A. C. M. Azeredo-Espin, A. M. L. Campinas State Univ UNICAMP, Mol Biol & Genet Engn Ctr CBMEG, BR-13083875 Campinas, SP, Brazilpt_BR
unicamp.authorAzeredo-Espin, A. M. L. Campinas State Univ UNICAMP, Dept Genet & Evolut DGE, Biol Inst IB, BR-13083875 Campinas, SP, Brazilpt_BR
dc.subjectInternal transcribed spacer 2pt_BR
dc.subjectSecondary structurept_BR
dc.subject.wosRibosomal-rna Genespt_BR
dc.subject.wosGenus Lucilia Dipterapt_BR
dc.subject.wosMitochondrial Genomept_BR
dc.subject.wosConcerted Evolutionpt_BR
dc.subject.wosBlowflies Calliphoridaept_BR
dc.subject.wosSpecies Identificationpt_BR
dc.subject.wosBayesian Phylogeneticspt_BR
dc.description.abstractThe internal transcribed spacer 2 (ITS2) is a small non-coding region located inside the nuclear ribosomal DNA cluster. ITS2 sequence variability is thought to be appropriate to differentiate species and for phylogenetic reconstructions analyses, which can be further improved if structural information is considered. We evaluated the potential of ITS2 as a molecular marker for phylogenetic inference in Calliphoridae (Diptera: Brachycera) using a broad range of inference methods and different substitution models, accounting or not for structural information. Sequence analyses revealed a hierarchically organized pattern of sequence variation and a small level of nucleotide substitution saturation. Intragenomic variation due to small sequence repeats was found mainly in the most variable domain (IV), but it has no significant impact on the phylogenetic signal at the species level. Inferred secondary structures revealed that GC pairs are more frequently found flanking bulges and loops regions in more conserved domains, thus ensuring structure stability. In the phylogenetic analyses, the use of substitution models accounting for structural information significantly improves phylogenetic inference in both neighbour-joining and Bayesian analyses, although the former provides limited resolution for dealing with highly divergent sequences. For Bayesian analyses, a significant improvement in likelihood was observed when considering structure information, although with small changes in topology and overall support, probably reflecting better evolutionary rates estimates. Based on these findings, ITS2 is a suitable molecular marker for phylogenetic analyses in Calliphoridae, at both species and generic
dc.identifier.citationGenetica. Springer, v. 139, n. 9, n. 1189, n. 1207, 2011.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberFAPESP [09/51723, 05/50100-5, 06/61217-3, 08/56769-2]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T17:29:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2011en
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